Antibodies, Proteins, Kits and Reagents for Life Science

Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] - BSA and Azide free (ab240409)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750) - BSA and Azide free
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] - BSA and Azide free
See all EGFR primary antibodies
Description

Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human lung carcinoma tissue. ICC/IF: HCC827 cells Flow Cyt: HCC827 cells
General notes
ab240409 is the carrier-free version of ab227737.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP111
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Processes

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] - BSA and Azide free (ab240409)

Immunocytochemistry/ Immunofluorescence analysis of HCC827(human lung adenocarcinoma epithelial cell), negative control:
A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:100 (4.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227680).
Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] - BSA and Azide free (ab240409)

Flow Cytometry analysis of HCC827(Human lung adenocarcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:400 dilution (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227680).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] - BSA and Azide free (ab240409)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for EGFR (deleted E746 + L747 + R748 + E749 + A750) using ab227737 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing tris buffered saline, BSA, and sodium azide (ab227737).
Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] - BSA and Azide free (ab240409)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com