Anti-EGFR antibody (ab245362)
Key features and details
- Rabbit polyclonal to EGFR
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EGFR antibody
See all EGFR primary antibodies -
Description
Rabbit polyclonal to EGFR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human EGFR aa 1100-1150. The exact sequence is proprietary.
Database link: P00533 -
Positive control
- IHC-P: Human placenta tissue. WB: HeLa and MCF7 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245362 was affinity purified using an epitope specific to EGFR immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody (ab245362)
Formalin-fixed, paraffin-embedded human placenta tissue stained for EGFR with ab245362 at a 1/1,000 dilution in immunohistochemical analysis. DAB staining.
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Western blot - Anti-EGFR antibody (ab245362)All lanes : Anti-EGFR antibody (ab245362) at 0.02 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 134 kDaPrepared using NETN lysis buffer.
Detection: Chemiluminescence.
