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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs

Anti-EEF1D antibody (ab228803)

Anti-EEF1D antibody (ab228803)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to EEF1D
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EEF1D antibody
    See all EEF1D primary antibodies
  • Description

    Rabbit polyclonal to EEF1D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human EEF1D (internal sequence). The exact sequence is proprietary.
    Database link: P29692

  • Positive control

    • WB: A549, THP-1, HL-60 and NCI-H929 whole cell lysate. IHC-P: Human adenocarcinoma tissue. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Western blot - Anti-EEF1D antibody (ab228803)
    Western blot - Anti-EEF1D antibody (ab228803)
    Anti-EEF1D antibody (ab228803) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 31 kDa



    10% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (ab228803)
    Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (ab228803)

    Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EEF1D (red) using ab228803 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1D antibody (ab228803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1D antibody (ab228803)

    Paraffin-embedded human adenocarcinoma tissue stained for EEF1D using ab228803 at 1/250 dilution in immunohistochemical analysis.

  • Western blot - Anti-EEF1D antibody (ab228803)
    Western blot - Anti-EEF1D antibody (ab228803)
    All lanes : Anti-EEF1D antibody (ab228803) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
    Lane 3 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 31 kDa



    10% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ICC/IF, WB

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