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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Endothelial

Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)

Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12451] to EDIL3/DEL1 - BSA and Azide free
  • Suitable for: ICC, IP, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free
    See all EDIL3/DEL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12451] to EDIL3/DEL1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, WB, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251027 is the carrier-free version of ab190692.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR12451
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors

Images

  • Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    All lanes : Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa



    This data was developed using ab190692, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    Immunocytochemistry - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    This data was developed using ab190692, the same antibody clone in a different buffer formulation.Immunofluorescent staining of 4% paraformaldehyde fixed Hela cells labeling EDIL3/DEL1 with ab190692 at 1/250 dilution (green) and DAPI nuclear staining (blue). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 488). The image also shows negative control cells.
  • Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    All lanes : Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa



    This data was developed using ab190692, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    Flow Cytometry - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    This data was developed using ab190692, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling EDIL3/DEL1 with ab190692 at 1/340 dilution (red) compared to a Rabbit monoclonal IgGcontrol antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC).
  • Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    This data was developed using ab190692, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of HeLa cell lysate labeling EDIL3/DEL1 with ab190692 at 1/50 dilution (lane 1) compared to a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.
  • Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)
    Anti-EDIL3/DEL1 antibody [EPR12451] - BSA and Azide free (ab251027)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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