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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIID

Anti-EDF1 antibody (ab174651)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-EDF1 antibody (ab174651)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to EDF1
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EDF1 antibody
  • Description

    Rabbit polyclonal to EDF1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human EDF1 aa 98-148. The exact sequence is proprietary. NP_003783.1.
    Sequence:

    KINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIEKGPRA K


    Database link: O60869
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab174651 was affinity purified using an epitope specific to EDF1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIID
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Signal Transduction
    • Metabolism
    • Lipid metabolism

Images

  • Western blot - Anti-EDF1 antibody (ab174651)
    Western blot - Anti-EDF1 antibody (ab174651)
    All lanes : Anti-EDF1 antibody (ab174651) at 0.100000001490116 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 16 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-EDF1 antibody (ab174651)
    Immunoprecipitation - Anti-EDF1 antibody (ab174651)

    Detection of EDF1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab174651 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab174651 was used at 1 µg/ml. Lane 2 represents control IgG IP. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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