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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway

Anti-EDAR antibody [EDAR12] (ab245684)

Anti-EDAR antibody [EDAR12] (ab245684)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EDAR12] to EDAR
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EDAR antibody [EDAR12]
    See all EDAR primary antibodies
  • Description

    Rabbit monoclonal [EDAR12] to EDAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to EDAR. ab245684 was prepared by immunizing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.

  • Epitope

    CRD1 of the extracellular domain of EDAR.
  • Positive control

    • ICC/IF: A549 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Proclin 300
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EDAR12
  • Isotype

    IgG
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EDAR antibody [EDAR12] (ab245684)
    Immunocytochemistry/ Immunofluorescence - Anti-EDAR antibody [EDAR12] (ab245684)

    PFA fixed, 0.15% Triton permeabilized A549 (Human lung carcinoma cell line) cells labeling EDAR using ab245684 at 10 µg/ml, 1 hr (green) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), in ICC/IF. Nuclear probe is visualized in blue (DAPI).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, WB

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