Anti-EBS antibody - N-terminal (ab224439)
Key features and details
- Rabbit polyclonal to EBS - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EBS antibody - N-terminal
See all EBS primary antibodies -
Description
Rabbit polyclonal to EBS - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human EBS aa 1-100 (N terminal).
Database link: Q9BTE0-2 -
Positive control
- WB: EBS over-expression - HEK-293T whole cell lysates; IHC-P: Human spleen tissue.
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General notes
This product was previously labelled as NAT9
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EBS antibody - N-terminal (ab224439)All lanes : Anti-EBS antibody - N-terminal (ab224439) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : EBS over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 23 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBS antibody - N-terminal (ab224439)Immunohistochemical analysis of human spleen tissue labeling EBS in the cytoplasm in subsets of cells in the red pulp with ab224439 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
