Anti-EBI3 antibody [EPR5747] (ab124694)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5747] to EBI3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EBI3 antibody [EPR5747]
See all EBI3 primary antibodies -
Description
Rabbit monoclonal [EPR5747] to EBI3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat -
Immunogen
Synthetic peptide within Human EBI3 aa 200 to the C-terminus. The exact sequence is proprietary.
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Positive control
- Rat and Human spleen, Human tonsil,K562 and JAR cell lysates; permeabilized K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5747 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified) + Human tonsil tissue lysate at 10 µg
Secondary
HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)
Lane 1 : K562 cell lysate
Lane 2 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/10000 dilution (unpurified)
Lane 1 : Rat spleen tissue lysate
Lane 2 : Human tonsil tissue lysate
Lane 3 : K562 cell lysate
Lane 4 : JAR cell lysate
Lane 5 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
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