Anti-IL-1 beta antibody (ab226918)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-1 beta antibody
See all IL-1 beta primary antibodies -
Description
Rabbit polyclonal to IL-1 beta -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IL-1 beta (internal sequence). The exact sequence is proprietary.
Database link: P01584 -
Positive control
- WB: THP-1 treated with LPS and ATP, whole cell extract. IHC-P: Human lung cancer tissue. IF: THP-1 treated with PMA and LPS, whole cell.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL-1 beta antibody (ab226918)All lanes : Anti-IL-1 beta antibody (ab226918) at 1/500 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg
Lane 2 : THP-1 (human monocytic leukemia cell line) treated with 500 ng/ml LPS for 4 hours and 1 mM ATP for 1.5 hours, whole cell extract at 30 µg
Lane 3 : Conditioned medium
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab226918)Paraffin-embedded human lung cancer tissue stained for IL-1 beta using ab226918 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. -
Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody (ab226918)Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) whole cells (Mock - Left or Treated - Right side) labelling IL-1 beta (green) using ab226918 (Top squares) at 1/500 dilution. Down squares: co-stained with phalloidin (red), a cytoskeleton marker, diluted at 1:100 and fluoroshield with DAPI (blue). Cells treated with 100 ng/ml PMA for 16 hours and 500 ng/ml LPS for 2 hours.
