Anti-EAAT4 antibody (ab41650)
Key features and details
- Rabbit polyclonal to EAAT4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-EAAT4 antibody
See all EAAT4 primary antibodies -
Description
Rabbit polyclonal to EAAT4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human EAAT4.
Read Abcam's proprietary immunogen policy (Peptide available as ab45367.) -
Positive control
- This antibody gave a positive signal in Mouse Cerebellum tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatDogHumanApplication Abreviews Notes WB Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa).Notes WB
Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa).Target
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Relevance
EAAT4 is expressed predominantly in the cerebellum (Purkinje cells) and weakly in the forebrain, where it transports L-glutamate, L-aspartate and D-aspartate, acting as a symport by cotransporting sodium. EAAT4 may function as a combined transporter and inhibitory glutamate receptor and also functions as an anion-selective ion channel. -
Cellular localization
Membrane; multi-pass membrane protein. -
Database links
- Entrez Gene: 403749 Dog
- Entrez Gene: 6511 Human
- Entrez Gene: 20513 Mouse
- Entrez Gene: 84012 Rat
- Omim: 600637 Human
- SwissProt: Q9N1R2 Dog
- SwissProt: P48664 Human
- SwissProt: O35544 Mouse
see all -
Alternative names
- Excitatory amino acid transporter 4 antibody
- High affinity neuronal glutamate transporter antibody
- MGC33092 antibody
see all
Images
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Western blot - Anti-EAAT4 antibody (ab41650)Anti-EAAT4 antibody (ab41650) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
References (3)
ab41650 has been referenced in 3 publications.
- Kim YS et al. Neuroprotective effects of magnesium L-threonate in a hypoxic zebrafish model. BMC Neurosci 21:29 (2020). PubMed: 32590943
- Zhou L et al. MicroRNA-128 Protects Dopamine Neurons from Apoptosis and Upregulates the Expression of Excitatory Amino Acid Transporter 4 in Parkinson's Disease by Binding to AXIN1. Cell Physiol Biochem 51:2275-2289 (2018). PubMed: 30537735
- Ramachandran PS et al. Nonallele Specific Silencing of Ataxin-7 Improves Disease Phenotypes in a Mouse Model of SCA7. Mol Ther N/A:N/A (2014). PubMed: 24930601
Images
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Western blot - Anti-EAAT4 antibody (ab41650)Anti-EAAT4 antibody (ab41650) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
