Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EAAT4 antibody (ab41650)

Key features and details

  • Rabbit polyclonal to EAAT4
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab41650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
All applications
Rat
Dog
Human
Application Abreviews Notes
WB
Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa).
Notes
WB
Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa).

Target

  • Relevance

    EAAT4 is expressed predominantly in the cerebellum (Purkinje cells) and weakly in the forebrain, where it transports L-glutamate, L-aspartate and D-aspartate, acting as a symport by cotransporting sodium. EAAT4 may function as a combined transporter and inhibitory glutamate receptor and also functions as an anion-selective ion channel.

  • Cellular localization

    Membrane; multi-pass membrane protein.

  • Database links

    see all

  • Alternative names

    • Excitatory amino acid transporter 4 antibody
    • High affinity neuronal glutamate transporter antibody
    • MGC33092 antibody
    • MGC43671 antibody
    • Sodium dependent glutamate/aspartate transporter antibody
    • Solute carrier family 1 (high affinity aspartate/glutamate transporter) member 6 antibody
    • Solute carrier family 1 member 6 antibody
    see all

Images

  • Western blot - Anti-EAAT4 antibody (ab41650)
    Western blot - Anti-EAAT4 antibody (ab41650)
    Anti-EAAT4 antibody (ab41650) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

References (3)

Publishing research using ab41650? Please let us know so that we can cite the reference in this datasheet.

ab41650 has been referenced in 3 publications.

  • Kim YS  et al. Neuroprotective effects of magnesium L-threonate in a hypoxic zebrafish model. BMC Neurosci 21:29 (2020). PubMed: 32590943
  • Zhou L  et al. MicroRNA-128 Protects Dopamine Neurons from Apoptosis and Upregulates the Expression of Excitatory Amino Acid Transporter 4 in Parkinson's Disease by Binding to AXIN1. Cell Physiol Biochem 51:2275-2289 (2018). PubMed: 30537735
  • Ramachandran PS  et al. Nonallele Specific Silencing of Ataxin-7 Improves Disease Phenotypes in a Mouse Model of SCA7. Mol Ther N/A:N/A (2014). PubMed: 24930601

Images

  • Western blot - Anti-EAAT4 antibody (ab41650)
    Western blot - Anti-EAAT4 antibody (ab41650)
    Anti-EAAT4 antibody (ab41650) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

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