Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [JAI-1-49-3] to Dysferlin
  • Suitable for: ICC/IF, IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Dysferlin antibody [JAI-1-49-3]
    See all Dysferlin primary antibodies

  • Description

    Rabbit monoclonal [JAI-1-49-3] to Dysferlin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-Fr
    Mouse
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Dysferlin aa 100-200. The exact sequence is proprietary.
    Database link: O75923

  • Positive control

    • WB: Human and mouse skeletal muscle tissue lysates. IHC-P: Human skeletal muscle tissue. IHC-Fr: Human and mouse skeletal muscle tissues. ICC/IF: A673 cells.

  • General notes

    This antibody was made in collaboration with the Jain Foundation whose goal is to hasten EVERY avenue that may lead to the cure for LGMD2B/Miyoshi.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 7.20 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    JAI-1-49-3

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Dysferlin with purified ab124684 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Dysferlin with unpurified ab124684 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)

    Immunohistochemistry (Frozen sections) analysis of unfixed frozen human skeletal muscle tissue (control) labelling Dysferlin with unpurified ab124684 at a dilution of 1/200.

  • Immunocytochemistry/ Immunofluorescence - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Immunocytochemistry/ Immunofluorescence - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)

    Immunocytochemistry/Immunofluorescence analysis of A673 cells labelling Dysferlin with purified ab124684 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Western blot - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Western blot - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Anti-Dysferlin antibody [JAI-1-49-3] (ab124684) at 1/1000 dilution (purified) + Mouse muscle tissue lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 237 kDa
    Observed band size: 280 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Western blot - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Anti-Dysferlin antibody [JAI-1-49-3] (ab124684) at 1/1000 dilution (purified) + Human skeletal muscle tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 237 kDa
    Observed band size: 280 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Western blot - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    All lanes : Anti-Dysferlin antibody [JAI-1-49-3] (ab124684) at 1/1000 dilution (unpurified)

    Lane 1 : Human skeletal muscle tissue lysate (control)
    Lane 2 : Human skeletal muscle tissue lysate (LGMD2B)
    Lane 3 : Mouse skeletal muscle tissue lysate (wild-type mice)
    Lane 4 : Mouse skeletal muscle tissue lysate (Dysf-/- transgenic mouse)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 237 kDa
    Observed band size: 280 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)

    Immunohistochemistry (Frozen sections) analysis of unfixed frozen mouse skeletal muscle tissue (wild type) labelling Dysferlin with unpurified ab124684 at a dilution of 1/200.

  • OI-RD Scanning - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    OI-RD Scanning - Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)
    Anti-Dysferlin antibody [JAI-1-49-3] (ab124684)

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