Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9053(2)(ABC)] to Dynamin 2 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free
    See all Dynamin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9053(2)(ABC)] to Dynamin 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249002 is the carrier-free version of ab151555. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249002 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    Immunocytochemistry - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)

    This data was developed using ab151555 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 cells labelling Dynamin 2 with ab151555 at 1/50 (19.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Western blot - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    Western blot - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    All lanes : Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] (ab151555) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 98 kDa



    This data was developed using ab151555, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    This data was developed using ab151555, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Dynamin 2 with ab151555 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    This data was developed using ab151555, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling Dynamin 2 with ab151555 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)
    Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] - BSA and Azide free (ab249002)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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