Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Drebrin antibody [EPR12640(B)] (ab176318)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12640(B)] to Drebrin
Suitable for: IP, WB, ICC/IF
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Drebrin antibody [EPR12640(B)]
See all Drebrin primary antibodies
Description

Rabbit monoclonal [EPR12640(B)] to Drebrin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Drebrin aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q16643
Positive control
- SH-SY5Y, Fetal brain, HeLa and Neuro-2a lysates, SH-SY5Y cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12640(B)
Isotype

IgG
Research areas

Western blot - Anti-Drebrin antibody [EPR12640(B)] (ab176318)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - ab176318 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab176318 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab176318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12640(B)] (ab176318)

Immunofluorescent staining of SH-SY5Y cells labeling Drebrin using ab176318 at a 1/100 dilution (green), with DAPI nuclear staining (blue).
Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] (ab176318)

Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate labeling Drebrin using ab176318 at a 1/10 dilution.
Western blot - Anti-Drebrin antibody [EPR12640(B)] (ab176318)

All lanes : Anti-Drebrin antibody [EPR12640(B)] (ab176318) at 1/1000 dilution

Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lane 3 : HeLa lysate
Lane 4 : Neuro-2a lysate

Predicted band size: 71 kDa
Anti-Drebrin antibody [EPR12640(B)] (ab176318)

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