Anti-Dppa2 antibody (ab91318)
Key features and details
- Rabbit polyclonal to Dppa2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Dppa2 antibody -
Description
Rabbit polyclonal to Dppa2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Dppa2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104208) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: IOUD2; F9; Mouse EG; E14Tg2a.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF MouseWB MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Relevance
Dppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 73703 Mouse
- SwissProt: Q9CWH0 Mouse
- Unigene: 27857 Mouse
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Alternative names
- cancer/testis antigen 100 antibody
- CT100 antibody
- Developmental Pluripotency Associated 2 antibody
see all
Images
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Western blot - Anti-Dppa2 antibody (ab91318)All lanes : Anti-Dppa2 antibody (ab91318) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Dppa2 antibody (ab91318)ICC/IF image of ab91318 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91318, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab91318, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (2)
ab91318 has been referenced in 2 publications.
- Yan YL et al. DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program. PLoS Biol 17:e3000324 (2019). PubMed: 31226106
- Tung PY et al. Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes. Stem Cells N/A:N/A (2013). WB, IP, ChIP . PubMed: 23963736
Images
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Western blot - Anti-Dppa2 antibody (ab91318)All lanes : Anti-Dppa2 antibody (ab91318) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Dppa2 antibody (ab91318)ICC/IF image of ab91318 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91318, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab91318, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.