Anti-DNAJC21 antibody (ab86434)
Key features and details
- Rabbit polyclonal to DNAJC21
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNAJC21 antibody
See all DNAJC21 primary antibodies -
Description
Rabbit polyclonal to DNAJC21 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 400-450 of Human DNAJC21 (NP_001012339.2)
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Positive control
- Whole cell lysate from HeLa and 293T cells.
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General notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNAJC21 antibody (ab86434)All lanes : Anti-DNAJC21 antibody (ab86434) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 62 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
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Immunoprecipitation - Anti-DNAJC21 antibody (ab86434)Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.
