Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNAJA4 antibody [EPR8756] (ab185553)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8756] to DNAJA4
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DNAJA4 antibody [EPR8756]
    See all DNAJA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR8756] to DNAJA4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain and HeLa whole cell lysate (ab150035); HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DNAJA4 antibody [EPR8756] (ab185553)
    Western blot - Anti-DNAJA4 antibody [EPR8756] (ab185553)
    All lanes : Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Hela cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    Blocking buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-DNAJA4 antibody [EPR8756] (ab185553)
    Flow Cytometry - Anti-DNAJA4 antibody [EPR8756] (ab185553)

    Flow cytometric analysis of DNAJA4 in 2% paraformaldehyde HeLa cells using ab185553 at 1/300 dilution (pink). Secondary antibody: Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green).

  • Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (ab185553)
    Immunoprecipitation - Anti-DNAJA4 antibody [EPR8756] (ab185553)

    Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2: PBS used instead of Human fetal brain lysates. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.  Blocking buffer: 5% NFDM/TBST.

  • Anti-DNAJA4 antibody [EPR8756] (ab185553)
    Anti-DNAJA4 antibody [EPR8756] (ab185553)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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