Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNAJA1 antibody [EPR7248] (ab126774)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7248] to DNAJA1
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-DNAJA1 antibody [EPR7248]
    See all DNAJA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7248] to DNAJA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DNAJA1 aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, Jurkat, HepG2, SKBR 3, Jurkat and Raji cell lysates. Flow Cyt: Jurkat cells.

  • General notes

     

     

     This product was previously labelled as HDJ2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 4.20 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7248

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : DNAJA1 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab126774 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab126774 Anti-DNAJA1 antibody [EPR7248] was shown to specifically react with DNAJA1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266437 (knockout cell lysate ab257925) was used.  Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE.  ab126774 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)

    Lane 1: Wild-type DNAJA1 whole cell lysate (20 µg)              
    Lane 2: DNAJA1 knockout  DNAJA1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126774 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab126774 was shown to recognize DNAJA1 in wild-type HAP1 samples along with additional cross-reactive bands. No band was observed when DNAJA1 knockout samples were examined. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE.  Ab126774 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    Flow Cytometry - Anti-DNAJA1 antibody [EPR7248] (ab126774)

    ab126774 at 1/10 dilution staining DNAJA1 in permeabilized Jurkat cells by Flow cytometry (red). Rabbit IgG negative control (green).

  • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : SKBR 3 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 45 kDa

  • OI-RD Scanning - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    OI-RD Scanning - Anti-DNAJA1 antibody [EPR7248] (ab126774)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-DNAJA1 antibody [EPR7248] (ab126774)
    Anti-DNAJA1 antibody [EPR7248] (ab126774)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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