Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

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Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-DNA polymerase mu antibody [EPR10470(B)]
See all DNA polymerase mu primary antibodies
Description

Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human DNA polymerase mu (UniProt Q9NP87).
Positive control
- WB: 293T, HeLa, Daudi and Jurkat cell lysates. IP: Jurkat cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10470(B)
Isotype

IgG
Research areas

Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)

All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLM knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : Daudi cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 55 kDa

Lanes 1-4: Merged signal (red and green). Green - ab157465 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.

ab157465 Anti-DNA polymerase mu antibody [EPR10470(B)] was shown to specifically react with POLM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265557 (knockout cell lysate ab258127) was used. Wild-type and POLM knockout samples were subjected to SDS-PAGE. ab157465 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)

All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 55 kDa
Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)

Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution + immunoprecipitation pellet from Jurkat cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 55 kDa
Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)

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