Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-DNA polymerase mu antibody [EPR10470(B)]
    See all DNA polymerase mu primary antibodies

  • Description

    Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human DNA polymerase mu (UniProt Q9NP87).

  • Positive control

    • WB: 293T, HeLa, Daudi and Jurkat cell lysates. IP: Jurkat cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10470(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
    Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
    All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : POLM knockout HeLa cell lysate
    Lane 3 : HEK-293 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab157465 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab157465 Anti-DNA polymerase mu antibody [EPR10470(B)] was shown to specifically react with POLM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265557 (knockout cell lysate ab258127) was used. Wild-type and POLM knockout samples were subjected to SDS-PAGE. ab157465 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
    Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
    All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 55 kDa

  • Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
    Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
    Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution + immunoprecipitation pellet from Jurkat cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 55 kDa

  • Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
    Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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