Anti-Dishevelled 3 antibody (ab93850)
Key features and details
- Rabbit polyclonal to Dishevelled 3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dishevelled 3 antibody
See all Dishevelled 3 primary antibodies -
Description
Rabbit polyclonal to Dishevelled 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Human Dishevelled 3 protein (ab114497) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Ovary (Human) Tissue Lysate; Heart (Human) Tissue Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 mg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Notes WB
Use a concentration of 1 mg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Target
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Function
May play a role in the signal transduction pathway mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1857 Human
- Entrez Gene: 13544 Mouse
- Entrez Gene: 303811 Rat
- Omim: 601368 Human
- SwissProt: Q92997 Human
- SwissProt: Q61062 Mouse
- Unigene: 388116 Human
- Unigene: 247259 Mouse
see all -
Alternative names
- Dishevelled 3 (homologous to Drosophila dsh) antibody
- Dishevelled 3 antibody
- Dishevelled 3, dsh homolog (Drosophila) antibody
see all
Images
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Western blot - Anti-Dishevelled 3 antibody (ab93850)All lanes : Anti-Dishevelled 3 antibody (ab93850) at 1 µg/ml
Lane 1 : Human ovary tissue lysate - total protein (ab30222)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Datasheets and documents
References (0)
ab93850 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Dishevelled 3 antibody (ab93850)All lanes : Anti-Dishevelled 3 antibody (ab93850) at 1 µg/ml
Lane 1 : Human ovary tissue lysate - total protein (ab30222)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
