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Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free
    See all Dishevelled 2 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Dishevelled 2 phosphorylated at Serine 143.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Dishevelled 2 (phospho S143). The exact sequence is proprietary.
    (Peptide available as ab173138)

  • General notes

     

    ab169950 is the carrier-free version of ab124933 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab169950 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR145
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic

Images

  • Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
    Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
    All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (purified)

    Lane 1 : Untreated F9 cell lysate
    Lane 2 : Alkaline phosphatase treated F9 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab124933, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
    Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
    All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution (purified)

    Lane 1 : Untreated HEK293 cell lysate
    Lane 2 : Alkaline phosphatase treated HEK293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    This data was developed using ab124933, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
    Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
    All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (unpurified)

    Lane 1 : F9 cell lysate untreated
    Lane 2 : F9 cell lysate treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 78 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    This data was developed using ab124933, the same antibody clone in a different buffer formulation.

     

  • Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
    Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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