Anti-DHX30 antibody (ab85687)
Key features and details
- Rabbit polyclonal to DHX30
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DHX30 antibody
See all DHX30 primary antibodies -
Description
Rabbit polyclonal to DHX30 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide, corresponding to a region between amino acids 350-400 of Human DHX30 (NP_619520.1)
-
Positive control
- HeLa whole cell lysate (ab150035), 293T cell lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DHX30 antibody (ab85687)All lanes : Anti-DHX30 antibody (ab85687) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 110 kDa, 238 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
-
Immunoprecipitation - Anti-DHX30 antibody (ab85687)Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.
