Anti-DHCR7 antibody (ab226409)
Key features and details
- Rabbit polyclonal to DHCR7
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHCR7 antibody
See all DHCR7 primary antibodies -
Description
Rabbit polyclonal to DHCR7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human -
Immunogen
Synthetic peptide within Human DHCR7 aa 1-50. The exact sequence is proprietary.
Database link: Q9UBM7 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab226409 has not performed satisfactorily whenused for WB of DHCR7 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of DHCR7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226409 was affinity purified using an epitope specific to DHCR7 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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DHCR7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226409 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226409 at 1 µg/ml.
Lane 1: ab226409 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.