Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
![Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)](https://www.abcam.com/ps/products/6/ab6322/Images/ab6322-338103-anti-desmin-antibody-de-u-10-cytoskeleton-marker-immunohistochemistry.jpg "Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)")
Key features and details
- Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
See all Desmin primary antibodies -
Description
Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHuman
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Immunogen
Full length native protein (purified) corresponding to Pig Desmin.
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Positive control
- IHC-P: Human skeletal muscle, Mouse cardiac muscle and rat colon tissue.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DE-U-10 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on mouse cardiac muscle.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)](https://www.abcam.com/ps/products/6/ab6322/Images/ab6322-338102-anti-desmin-antibody-de-u-10-cytoskeleton-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on smooth muscle of rat colon.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)](https://www.abcam.com/ps/products/6/ab6322/Images/ab6322-12254-anti-desmin-antibody-de-u-10-cytoskeleton-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
