Anti-Desmin antibody (ab8592)
Key features and details
- Rabbit polyclonal to Desmin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Desmin antibody
See all Desmin primary antibodies -
Description
Rabbit polyclonal to Desmin -
Host species
Rabbit -
Specificity
In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Human Desmin.
Database link: P17661 -
General notes
Prolonged fixation in buffered formalin can destroy the epitope.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.01% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Desmin antibody (ab8592)All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution
Lane 1 : RMS13 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human heart tissue lysate
Lane 5 : C2C12 cell lysate
Lane 6 : Mouse skeletal muscle tissue lysate
Lane 7 : Mouse heart tissue lysate
Lane 8 : L6 cell lysate
Lane 9 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
