Anti-DERL1/Derlin-1 antibody (ab176732)
Key features and details
- Rabbit polyclonal to DERL1/Derlin-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DERL1/Derlin-1 antibody
See all DERL1/Derlin-1 primary antibodies -
Description
Rabbit polyclonal to DERL1/Derlin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Non human primates
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Immunogen
Synthetic peptide within Human DERL1/Derlin-1 aa 201-251. The exact sequence is proprietary. NP_077271.1.
Database link: Q9BUN8 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 cell lysates. IP: HeLa cell lysate. IHC-P: Human ovarian carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176732 is affinity purified using an epitope specific to DERL1/Derlin-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DERL1/Derlin-1 antibody (ab176732)All lanes : Anti-DERL1/Derlin-1 antibody (ab176732) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-DERL1/Derlin-1 antibody (ab176732)ab176732 at 0.4 µg/ml detecting DERL1/Derlin-1 in HeLa whole cell lysate by WB following IP. ab176732 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DERL1/Derlin-1 antibody (ab176732)Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling DERL1/Derlin-1 using ab176732 at a 1/200 dilution, followed by peroxidase substrate detection.
