Anti-Dengue Virus NS1 glycoprotein antibody [FB10.C12.E5.E9] (ab253233)
![Anti-Dengue Virus NS1 glycoprotein antibody [FB10.C12.E5.E9] (ab253233)](https://www.abcam.com/ps/products/253/ab253233/Images/ab253233-343669-AntiDengue-Virus-NS1-glycoprotein-antibody-FB10C12E5E9-elisa.jpg "Anti-Dengue Virus NS1 glycoprotein antibody [FB10.C12.E5.E9] (ab253233)")
Key features and details
- Mouse monoclonal [FB10.C12.E5.E9] to Dengue Virus NS1 glycoprotein
- Suitable for: ELISA
- Reacts with: Dengue virus 4
- Isotype: IgG1
Overview
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Product name
Anti-Dengue Virus NS1 glycoprotein antibody [FB10.C12.E5.E9]
See all Dengue Virus NS1 glycoprotein primary antibodies -
Description
Mouse monoclonal [FB10.C12.E5.E9] to Dengue Virus NS1 glycoprotein -
Host species
Mouse -
Specificity
This antibody is specific for the NS1 protein of Dengue virus serotype 4, and does not cross-react with NS1 from other Dengue virus serotypes. It also demonstrates negligible cross-reactivity with NS1 protein from Zika virus or other flaviviruses. No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Dengue virus 4 -
Immunogen
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Dengue virus 1 Dengue Virus NS1 glycoprotein.
Recombinant full length protein corresponding to Dengue virus 2 Dengue Virus NS1 glycoprotein.
Recombinant full length protein corresponding to Dengue virus 3 Dengue Virus NS1 glycoprotein.
Recombinant full length protein corresponding to Dengue virus 4 Dengue Virus NS1 glycoprotein. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
FB10.C12.E5.E9 -
Isotype
IgG1 -
Light chain type
lambda -
Research areas
Images
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ELISA - Anti-Dengue Virus NS1 glycoprotein antibody [FB10.C12.E5.E9] (ab253233)
ELISA plates coated on bench overnight in 0.1M carbonate pH 9.6, 100 µl/well, washed once in wash buffer 300 µl/well (TBS + 0.1% tween 20) and blocked 2 hrs in 1% BSA in D-PBS 300 µl/well. Antibodies diluted to working strength in diluent (DPBS + 1% BSA + 0.05% Tween 20 + 0.2% Proclin 950). Added at 100 µl/well and incubated 2 hrs shaken at ambient temperature. Washed 3 x 300 µl/well. Goat anti Mouse IgG-HRP diluted 1/2500 in diluent, added at 100 µl/well and incubated with shaking 1 hr at ambient. Plate washed 6X300 µl/well. TMB added at 100 µl/well. Reaction for screening assay stopped by addition of 1M HCl 100 µl/well.