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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)

Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7492] to DEF6 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-DEF6 antibody [EPR7492] - BSA and Azide free
    See all DEF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR7492] to DEF6 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248188 is the carrier-free version of ab126792 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248188 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 2.01 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7492
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other

Images

  • Western blot - Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
    Western blot - Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
    All lanes : Anti-DEF6 antibody [EPR7492] (ab126792) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Ramos lysate
    Lane 3 : MOLT4 lysate
    Lane 4 : Human thymus lysate
    Lane 5 : Jurkat lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 74 kDa



    This data was developed using ab126792, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
    Flow Cytometry - Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
    This data was developed using ab126792, the same antibody clone in a different buffer formulation.ab126792 at 1/100 dilution staining DEF6 in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
  • OI-RD Scanning - Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
    OI-RD Scanning - Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
    This data was developed using ab126792, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)
    Anti-DEF6 antibody [EPR7492] - BSA and Azide free (ab248188)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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