Anti-DDX42 antibody (ab89075)
Key features and details
- Mouse polyclonal to DDX42
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DDX42 antibody -
Description
Mouse polyclonal to DDX42 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Full length protein corresponding to Human DDX42 aa 1-819.
Sequence:MAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPT AGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYN EHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIR KSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQK ELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQ AKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEY QVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEA NEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANN LKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSI KTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNL EGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLG SENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLS NQ KAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPG FGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRG SSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHG GSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSK TDKTADGFAVPEPPKRKKSRWDS
Database link: Q86XP3-2 -
Positive control
- A431 cell lysate. Cell lysate from transfected 293T cells. HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DDX42 antibody (ab89075)Anti-DDX42 antibody (ab89075) at 1 µg/ml + A431 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~130 kDa why is the actual band size different from the predicted?
-
Western blot - Anti-DDX42 antibody (ab89075)All lanes : Anti-DDX42 antibody (ab89075) at 1 µg/ml
Lane 1 : cell lysate from DDX42 transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~130 kDa why is the actual band size different from the predicted?
-
Immunocytochemistry/ Immunofluorescence - Anti-DDX42 antibody (ab89075)ab89075 at 10 µg/ml staining DDX42 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.