Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)
![Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-1-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-human-testis.jpg "Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24148-58] to DDX4 / MVH
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DDX4 / MVH antibody [EPR24148-58]
See all DDX4 / MVH primary antibodies -
Description
Rabbit monoclonal [EPR24148-58] to DDX4 / MVH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human testis tissue lysate; Mouse testis tissue lysate; rat testis tissue lysate. IHC-P: Human testis and overy tissue; Mouse testis and ovary tissue; Rat testis and ovary tissue. IHC-Fr: Mouse testis tissue; Rat testis tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24148-58 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of human testis (PMID:26444630). The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-7-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-frozen-mouse-testis.jpg)
Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse testis is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-445435-anti-ddx4mvh-antibody-epr2414858-western-blot-lysate.jpg)
Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)All lanes : Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human spleen tissue lysate
Lane 3 : Human colon tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Human spleen, Human colon (PMID: 10920202).
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-3-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of mouse testis. The section was incubated with ab270534 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-8-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-frozen-rat-testis.jpg)
Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat testis is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-2-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-human-ovary.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of human ovary (PMID:26444630).The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-445436-anti-ddx4mvh-antibody-epr2414858-western-blot-lysate1.jpg)
Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)All lanes : Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: lane 1: 10 seconds lane 2: 7 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-4-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-mouse-ovary.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of mouse ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-5-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-rat-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of rat testis. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-6-anti-ddx4mvh-antibody-epr2414858-immunohistochemistry-rat-ovary.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of rat ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)](https://www.abcam.com/ps/products/270/ab270534/Images/ab270534-447404-benefitsofrecombinantantibodies.png)
Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)
