Anti-DDX27 antibody [2251C2a] (ab50696)
![Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab506962.gif "Anti-DDX27 antibody [2251C2a] (ab50696)")
Key features and details
- Mouse monoclonal [2251C2a] to DDX27
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-DDX27 antibody [2251C2a]
See all DDX27 primary antibodies -
Description
Mouse monoclonal [2251C2a] to DDX27 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant human DDX27
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Positive control
- HeLa cells and HeLa and NIH3T3 cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50696 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
2251C2a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa
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![Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab50696wb2.gif)
Western blot - Anti-DDX27 antibody [2251C2a] (ab50696)Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + NIH3T3 whole cell lyaste at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa
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![Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)](https://www.abcam.com/ps/products/50/ab50696/Images/ab50696icc.gif)
Immunocytochemistry - Anti-DDX27 antibody [2251C2a] (ab50696)ab50696 at 1/5 dilution staining human DDX27 in HeLa cells by Immunocytochemistry. A 1/200 dilution of AlexaFluor488 Goat Anti-mouse IgG was used as secondary.
