Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13807(B)] to DDX17 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free
    See all DDX17 primary antibodies

  • Description

    Rabbit monoclonal [EPR13807(B)] to DDX17 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, NIH/3T3, J774A.1, RAW264.7, C2C12 and HeLa cell lysates; Mouse embryo lysate.

  • General notes

    ab250190 is the carrier-free version of ab180190 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250190 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13807(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution

    Lane 1 : HeLa (Human colorectal adenocarcinoma epithelial cell) cell lysate
    Lane 2 : Mouse embryo lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
    Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cell lysate
    Lane 5 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate
    Lane 6 : C2C12 (Mouse myoblasts myoblast) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    This data was developed using ab180190, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 6 seconds.

  • Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/5000 dilution

    Lane 1 : Wild-type HEK 293 whole cell lysate
    Lane 2 : DDX17 knockout HEK 293 whole cell lysate
    Lane 3 : Hela whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 72 kDa



    This data was developed using ab180190, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab180190 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180190 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab180190 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 72 kDa



    This data was developed using ab180190, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Immunocytochemistry - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    This data was developed using ab180190, the same antibody clone in a different buffer formulation.ab180190 staining DDX17 antibody in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain. Negative control 1:PBS only.
  • Immunocytochemistry - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Immunocytochemistry - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    This data was developed using ab180190, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells labeling DDX17 with ab180190 at 1/100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    This data was developed using ab180190, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast tissue labeling DDX17 with ab180190 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Flow Cytometry - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    This data was developed using ab180190, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized MCF7 cells labeling DDX17 with ab180190 at 1/10 (red) or a rabbit IgG negative (green).
  • Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Western blot - Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution

    Lane 1 : HeLa (Human colorectal adenocarcinoma epithelial cell) cell lysate
    Lane 2 : Mouse embryo lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
    Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cell lysate
    Lane 5 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate
    Lane 6 : C2C12 (Mouse myoblasts myoblast) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     

     Exposure time: 6 s

  • Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)
    Anti-DDX17 antibody [EPR13807(B)] - BSA and Azide free (ab250190)

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