Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23547-2] to DCTN1/p150-glued
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DCTN1/p150-glued antibody [EPR23547-2]
    See all DCTN1/p150-glued primary antibodies

  • Description

    Rabbit monoclonal [EPR23547-2] to DCTN1/p150-glued

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain and cerebellum tissue lysates; Mouse brain and cerebellum tissue lysates; Rat brain tissue lysate. IP: Human brain tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)
    Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)
    All lanes : Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human large intestine tissue lysate
    Lane 3 : Human cerebellum tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse cerebellum tissue lysate
    Lane 6 : Rat brain tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 8856662, 27365401).

    Negative control: Human intestine (Roodink, I. (2009). Tackling the vascular heterogeneity issue in tumors: identification of novel targets for tumor therapy (Doctoral dissertation)).

    Lane 3 blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)
    Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)

    DCTN1/p150-glued was immunoprecipitated from 0.35 mg human brain tissue lysate with ab246505 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246505 at 1/1000 dilution (0.573 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human brain tissue lysate 10µg

    Lane 2: ab246505 IP in human brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246505 in human brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID: 8856662).

  • Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)
    Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)

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