Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DCAF13 antibody [EPR18723] (ab195121)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18723] to DCAF13
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-DCAF13 antibody [EPR18723]
See all DCAF13 primary antibodies
Description

Rabbit monoclonal [EPR18723] to DCAF13
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: C6, PC-12, NIH/3T3, Neuro-2a, F9, HeLa and 293T whole cell lysates; Mouse kidney and spleen lysates; Rat spleen lysate; Human fetal liver, fetal heart and fetal kidney lysates. IHC-P: Mouse ovary and colon tissues. IP: NIH/3T3 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18723
Isotype

IgG
Research areas

Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)

All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 49 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)

All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)

All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293T (Human epithelial cell line from embryonic kidney) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)

All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAF13 antibody [EPR18723] (ab195121)

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling DCAF13 with ab195121 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Mainly nuclear staining on mouse ovary is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAF13 antibody [EPR18723] (ab195121)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling DCAF13 with ab195121 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear/nucleolus staining with additional cytoplasm staining on mouse colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-DCAF13 antibody [EPR18723] (ab195121)

DCAF13 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab195121 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab195121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).

Lane 2: ab195121 IP in NIH/3T3 whole cell lysate.

Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab195121 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-DCAF13 antibody [EPR18723] (ab195121)

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