Anti-DCAF13 antibody [EPR18723] (ab195121)
![Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-256901-anti-dcaf13-antibody-epr18723-western-blot.jpg "Anti-DCAF13 antibody [EPR18723] (ab195121)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18723] to DCAF13
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DCAF13 antibody [EPR18723]
See all DCAF13 primary antibodies -
Description
Rabbit monoclonal [EPR18723] to DCAF13 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseIP MouseWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6, PC-12, NIH/3T3, Neuro-2a, F9, HeLa and 293T whole cell lysates; Mouse kidney and spleen lysates; Rat spleen lysate; Human fetal liver, fetal heart and fetal kidney lysates. IHC-P: Mouse ovary and colon tissues. IP: NIH/3T3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18723 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-256900-anti-dcaf13-antibody-epr18723-western-blot.jpg)
Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-256899-anti-dcaf13-antibody-epr18723-western-blot.jpg)
Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293T (Human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-256898-anti-dcaf13-antibody-epr18723-western-blot.jpg)
Western blot - Anti-DCAF13 antibody [EPR18723] (ab195121)All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-256897-anti-dcaf13-antibody-epr18723-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAF13 antibody [EPR18723] (ab195121)Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling DCAF13 with ab195121 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Mainly nuclear staining on mouse ovary is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-256896-anti-dcaf13-antibody-epr18723-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAF13 antibody [EPR18723] (ab195121)Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling DCAF13 with ab195121 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear/nucleolus staining with additional cytoplasm staining on mouse colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-256895-anti-dcaf13-antibody-epr18723-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DCAF13 antibody [EPR18723] (ab195121)DCAF13 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab195121 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab195121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).
Lane 2: ab195121 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab195121 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-DCAF13 antibody [EPR18723] (ab195121)](https://www.abcam.com/ps/products/195/ab195121/Images/ab195121-8-benefits-of-recombinant-antibodies.png)
Anti-DCAF13 antibody [EPR18723] (ab195121)
