Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DARC antibody [EPR5205] (ab137044)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5205] to DARC
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DARC antibody [EPR5205]
    See all DARC primary antibodies

  • Description

    Rabbit monoclonal [EPR5205] to DARC

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues within the intracellular domain of Human DARC (UniProt ID: Q16570)

  • Positive control

    • WB: Human fetal liver, Human fetal kidney, Human tonsil, kidney, stomach, TF 1 and K562 lysates; IHC-P: Human kidney tissue IP: TF-1 cells

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1:500 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)
    Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)

    ab137044 (purified) at 1:30 dilution (2µg) immunoprecipitating DARC in TF-1 whole cell lysate.
    Lane 1 (input): TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10µg
    Lane 2 (+): ab137044 & TF-1 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
    Western blot - Anti-DARC antibody [EPR5205] (ab137044)
    All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/2000 dilution (Purified)

    Lane 1 : Human kidney lysates at 15 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
    Western blot - Anti-DARC antibody [EPR5205] (ab137044)
    All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution (Purified)

    Lane 1 : Human stomach lysates at 15 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.

    This image was generated using the unpurified version of the product. 

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
    Western blot - Anti-DARC antibody [EPR5205] (ab137044)
    All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human tonsil lysate
    Lane 4 : TF 1 lysate
    Lane 5 : K562 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 35 kDa



    This image was generated using the unpurified version of the product. 

  • Anti-DARC antibody [EPR5205] (ab137044)
    Anti-DARC antibody [EPR5205] (ab137044)

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