Anti-IgA Secretory Component antibody [SC-05] (ab3924)
![Anti-IgA Secretory Component antibody [SC-05] (ab3924)](https://www.abcam.com/ps/products/3/ab3924/Images/ab3924-8412-anti-iga-secretory-component-antibody-sc-05-western-blot.jpg "Anti-IgA Secretory Component antibody [SC-05] (ab3924)")
Key features and details
- Mouse monoclonal [SC-05] to IgA Secretory Component
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IgA Secretory Component antibody [SC-05]
See all IgA Secretory Component primary antibodies -
Description
Mouse monoclonal [SC-05] to IgA Secretory Component -
Host species
Mouse -
Specificity
ab3924 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). The recognized glycoprotein is a specific membrane marker of glandular carcinomas. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgA Secretory Component. Affinity-purified secretory component from human colostrum.
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Positive control
- WB: MCF7 and MDA-MB-361 cell lysates.
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General notes
Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Primary antibody notes
Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland. -
Clonality
Monoclonal -
Clone number
SC-05 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924)All lanes : Anti-IgA Secretory Component antibody [SC-05] (ab3924) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Secretory Component Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
