Anti-DAP12 antibody (ab93846)
Key features and details
- Rabbit polyclonal to DAP12
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DAP12 antibody
See all DAP12 primary antibodies -
Description
Rabbit polyclonal to DAP12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human DAP12 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab107906) -
Positive control
- This antibody gave a positive signal in the follwoing lysates: Lung (Human) Tissue Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications SheepCowDogApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).ICC/IF Use a concentration of 10 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).ICC/IF
Use a concentration of 10 µg/ml.Target
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Function
Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. -
Tissue specificity
Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum. -
Involvement in disease
Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Belongs to the TYROBP family. -
Post-translational
modificationsTyrosine phosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 282390 Cow
- Entrez Gene: 476477 Dog
- Entrez Gene: 7305 Human
- Entrez Gene: 443208 Sheep
- Omim: 604142 Human
- SwissProt: Q95J79 Cow
- SwissProt: O43914 Human
- Unigene: 515369 Human
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Alternative names
- DAP 12 antibody
- DAP12 antibody
- DNAX activation protein 12 antibody
see all
Images
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Western blot - Anti-DAP12 antibody (ab93846)Anti-DAP12 antibody (ab93846) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The DAP12 protein sequence contains a 21-residue signal sequence at the N-terminus. Cleavage of this peptide may account for the doublet observed. -
Immunocytochemistry/ Immunofluorescence - Anti-DAP12 antibody (ab93846)ICC/IF image of ab93846 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93846, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab93846, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 10µg/ml.
Protocols
Datasheets and documents
References (1)
ab93846 has been referenced in 1 publication.
- Raha AA et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . PubMed: 27662313
Images
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Western blot - Anti-DAP12 antibody (ab93846)Anti-DAP12 antibody (ab93846) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The DAP12 protein sequence contains a 21-residue signal sequence at the N-terminus. Cleavage of this peptide may account for the doublet observed. -
Immunocytochemistry/ Immunofluorescence - Anti-DAP12 antibody (ab93846)ICC/IF image of ab93846 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93846, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab93846, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 10µg/ml.
