Anti-Cytokeratin antibody [34BE12] (ab191208)
![Anti-Cytokeratin antibody [34BE12] (ab191208)](https://www.abcam.com/ps/products/191/ab191208/Images/ab191208-227993-ab191208b.jpg "Anti-Cytokeratin antibody [34BE12] (ab191208)")
Key features and details
- Mouse monoclonal [34BE12] to Cytokeratin
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin antibody [34BE12]
See all Cytokeratin primary antibodies -
Description
Mouse monoclonal [34BE12] to Cytokeratin -
Host species
Mouse -
Specificity
ab191208 recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. ab191208 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Other Immunogen Type corresponding to Human Cytokeratin. (Solubilized keratin extract from Human stratum corneum).
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Positive control
- IHC-P: Human prostate carcinoma tissue. ICC: HeLa cells. WB: MCF7 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
Purification notes
purified from Bioreactor Concentrates by Protein A/G column chromatography -
Clonality
Monoclonal -
Clone number
34BE12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (ab191208)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Cytokeratin with ab191208 at 1 µg/ml.
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![Immunocytochemistry - Anti-Cytokeratin antibody [34BE12] (ab191208)](https://www.abcam.com/ps/products/191/ab191208/Images/ab191208-453890-anti-cytokeratin-antibody-34be12-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-Cytokeratin antibody [34BE12] (ab191208)Immunocytochemistry analysis of MeOH-fixed HeLa (Human epithlial cell line from cervix adenocarcinoma) cells labeling Cytokeratin with ab191208 at 1 ug/ml.
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![Western blot - Anti-Cytokeratin antibody [34BE12] (ab191208)](https://www.abcam.com/ps/products/191/ab191208/Images/ab191208-453891-anti-cytokeratin-antibody-34be12-western-blot-mcf7-human.jpg)
Western blot - Anti-Cytokeratin antibody [34BE12] (ab191208)Anti-Cytokeratin antibody [34BE12] (ab191208) at 2 µg/ml + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 66 kDaWestern blot analysis of MCF-7 cells labeling Cytokeratin with ab191028 at 2 ug/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (ab191208)](https://www.abcam.com/ps/products/191/ab191208/Images/ab191208-227992-ab191208.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [34BE12] (ab191208)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Cytokeratin with ab191208 at 1 µg/ml.
