Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1619Y] to Cytokeratin 7 - BSA and Azide free
  • Suitable for: ICC, IP, IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free
    See all Cytokeratin 7 primary antibodies

  • Description

    Rabbit monoclonal [EPR1619Y] to Cytokeratin 7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bladder carcinoma tissue.

  • General notes

    Ab181831 is the carrier-free version of ab68459. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab181831 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
    Immunocytochemistry - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)

    Clone EPR1619Y (ab181831) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 647). Please refer to ab192077 for protocol details.

    ab192077 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192077 at a working dilution of 1 in 100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    This product gave a positive signal in 100% methanol (5 min) fixed T47D cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Cytokeratin 7 with purified ab68459 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68459).

     

  • Immunocytochemistry - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
    Immunocytochemistry - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)

    Clone EPR1619Y (ab181831) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (PE). Please refer to ab210628 for protocol details.

    Figure Legend for the image: ab210628 staining Cytokeratin 7 in T74D cells. The cells were fixed with 100%methanol, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210628 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in T74D cells fixed with 4%formaldehyde.

  • Immunocytochemistry - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
    Immunocytochemistry - Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)

    Clone EPR1619Y (ab181831) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (Alexa Fluor® 488). Please refer to ab185048 for protocol details.

    ab185048 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab185048 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    This product gave a positive signal in 100% methanol (5 min) fixed A549 cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
    Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)

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