Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
![Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.com/ps/products/7/ab7797/Images/ab7797-332236-anti-cytokeratin-18-antibody-dc-10-immunohistochemistry.jpg "Anti-Cytokeratin 18 antibody [DC 10] (ab7797)")
Key features and details
- Mouse monoclonal [DC 10] to Cytokeratin 18
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 18 antibody [DC 10]
See all Cytokeratin 18 primary antibodies -
Description
Mouse monoclonal [DC 10] to Cytokeratin 18 -
Host species
Mouse -
Specificity
Specific reactivity for the cytokeratin 18 (45 kD) polypeptide. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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Immunogen
PMC-42 human breast carcinoma cells.
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Positive control
- IHC-P: Colon carcinoma WB, ELISA, IP: MCF-7 or HeLa cell lysate
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General notes
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS -
Primary antibody notes
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. -
Clonality
Monoclonal -
Clone number
DC 10 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml
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![Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.com/ps/products/7/ab7797/Images/ab7797-332235-anti-cytokeratin-18-antibody-dc-10-western-blot.jpg)
Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.com/ps/products/7/ab7797/Images/ab7797-13262-anti-cytokeratin-18-antibody-dc-10-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
