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Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free (ab250241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14479(B)] to Cytochrome P450 Reductase - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free
    See all Cytochrome P450 Reductase primary antibodies

  • Description

    Rabbit monoclonal [EPR14479(B)] to Cytochrome P450 Reductase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HCT116, HepG2, A431 and HeLa cell lysates.

  • General notes

    Ab250241 is the carrier-free version of ab180597. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250241 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free (ab250241)
    Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free (ab250241)
    All lanes : Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/1000 dilution

    Lane 1 : Wild-type HCT116 cell lysate
    Lane 2 : POR knockout HCT116 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab180597).

    Lanes 1- 4: Merged signal (red and green). Green - ab180597 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab180597 was shown to react with Cytochrome P450 Reductase in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266889 (knockout cell lysate ab257596) was used. Wild-type HCT116 and POR knockout HCT116 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180597 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free (ab250241)
    Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free (ab250241)
    All lanes : Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/10000 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : Cytochrome P450 Reductase knockout HeLa lysate
    Lane 3 : HepG2 lysate
    Lane 4 : A431 lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 76 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab180597).

    Lanes 1-4: Merged signal (red and green). Green - ab180597 observed at 75 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab180597 Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] was shown to specifically react with Cytochrome P450 Reductase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264996 (knockout cell lysate ab257595) was used. Wild-type and Cytochrome P450 Reductase knockout samples were subjected to SDS-PAGE. ab180597 and Anti-GAPDH antibody [6C5] - Loading Control?(ab8245) were incubated overnight at 4^°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free (ab250241)
    Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free (ab250241)

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