Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3931] to Cystatin-B - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free
See all Cystatin-B primary antibodies -
Description
Rabbit monoclonal [EPR3931] to Cystatin-B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, U87-MG and JAR cell lysates; Human bladder tissue lysate. IHC-P: Human colon and kidney tissues.
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General notes
ab247554 is the carrier-free version of ab92449 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247554 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as Stefin B
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3931 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CSTB knockout HEK293T cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 11 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?This data was developed using ab92449, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab92449 observed at 15 kDa. Red - loading control ab8245 observed at 36 kDa.
ab92449 Anti-Cystatin-B antibody [EPR3931] was shown to specifically react with Cystatin-B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266321 (knockout cell lysate ab257905) was used. Wild-type and Cystatin-B knockout samples were subjected to SDS-PAGE. ab92449 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Human bladder tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDaThis data was developed using ab92449, the same antibody clone in a different buffer formulation.
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