Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3931] to Cystatin-B - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free
    See all Cystatin-B primary antibodies

  • Description

    Rabbit monoclonal [EPR3931] to Cystatin-B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, U87-MG and JAR cell lysates; Human bladder tissue lysate. IHC-P: Human colon and kidney tissues.

  • General notes

    ab247554 is the carrier-free version of ab92449 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247554 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as Stefin B

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    Western blot - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : CSTB knockout HEK293T cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 11 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab92449, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab92449 observed at 15 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab92449 Anti-Cystatin-B antibody [EPR3931] was shown to specifically react with Cystatin-B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266321 (knockout cell lysate ab257905) was used. Wild-type and Cystatin-B knockout samples were subjected to SDS-PAGE. ab92449 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    Western blot - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : Human bladder tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 11 kDa



    This data was developed using ab92449, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    This data was developed using ab92449, the same antibody clone in a different buffer formulation.ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    This data was developed using ab92449, the same antibody clone in a different buffer formulation.ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)
    Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free (ab247554)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.