Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CYP46A1 antibody [1A7] (ab244241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1A7] to CYP46A1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CYP46A1 antibody [1A7]
    See all CYP46A1 primary antibodies

  • Description

    Mouse monoclonal [1A7] to CYP46A1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat brain lysate. IHC-P: Mouse cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CYP46 antibody [1A7] (ab244241)
    Western blot - Anti-CYP46 antibody [1A7] (ab244241)
    Lane 1 : Anti-CYP46A1 antibody [1A7] (ab244241) at 0.43 µl
    Lane 2 : Anti-CYP46A1 antibody [1A7] (ab244241) at 0.43 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP46 antibody [1A7] (ab244241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP46 antibody [1A7] (ab244241)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CYP46 with ab244241 at 8.6µg/ml followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum. The section was incubated with ab244241 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-CYP46A1 antibody [1A7] (ab244241)
    Anti-CYP46A1 antibody [1A7] (ab244241)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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