Anti-CYP2U1 antibody (ab65128)
Key features and details
- Rabbit polyclonal to CYP2U1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP2U1 antibody
See all CYP2U1 primary antibodies -
Description
Rabbit polyclonal to CYP2U1 -
Host species
Rabbit -
Specificity
Detects endogenous levels of total CYP2U1 protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide (Human) from an internal sequence
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Positive control
- HeLa and LOVO cell extracts
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CYP2U1 antibody (ab65128)All lanes : Anti-CYP2U1 antibody (ab65128) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : LOVO cell extract
Lane 3 : LOVO cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-CYP2U1 antibody (ab65128)ICC/IF image of ab65128 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65128, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
