Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CYP2D6 antibody [EPR17868] (ab185625)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17868] to CYP2D6
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-CYP2D6 antibody [EPR17868]
See all CYP2D6 primary antibodies
Description

Rabbit monoclonal [EPR17868] to CYP2D6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: CYP2D6 transfected 293T lysate; Human fetal liver lysate; HeLa, K562, HepG2 and HT-29 whole cell lysates. IHC-P: Human liver, kidney and hepatocellular carcinoma tissues. ICC/IF: HT-29 cells. Flow Cyt: HT-29 cells.
General notes
This product was previously labelled as Cytochrome P450 2D6

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17868
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling CYP2D6 with ab185625 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic staining on HT-29 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab185625 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CYP2D6 with ab185625 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human liver is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CYP2D6 with ab185625 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human kidney is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling CYP2D6 with ab185625 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Flow cytometric analysis of 2% paraformaldehyde-fixed HT-29 (Human colorectal adenocarcinoma cells) cells labeling CYP2D6 with ab185625 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

All lanes : Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/10000 dilution

Lane 1 : Full-length CYP2D6 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 81 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Full-length CYP2D6 293T overexpressed lysates contain GFP tagged-aa1-497.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/10000 dilution + Human fetal liver lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 50,55 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, ab185625 recognizes two isoforms with the predicted MWs of 55kDa and 50kDa respectively.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

All lanes : Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/1000 dilution + HT-29 (Human colorectal adenocarcinoma cells) whole cell lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CYP2D6 antibody [EPR17868] (ab185625)

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