Anti-Cyclin E1 antibody [HE12] (ab3927)
![Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.com/ps/products/3/ab3927/Images/ab3927-319310-anti-cyclin-e1-antibody-he12-western-blot.jpg "Anti-Cyclin E1 antibody [HE12] (ab3927)")
Key features and details
- Mouse monoclonal [HE12] to Cyclin E1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin E1 antibody [HE12]
See all Cyclin E1 primary antibodies -
Description
Mouse monoclonal [HE12] to Cyclin E1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Cyclin E1.
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Positive control
- MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
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General notes
This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
HE12 -
Myeloma
unknown -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDaLanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.com/ps/products/3/ab3927/Images/ab3927-8419-anti-cyclin-e1-antibody-he12-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927) Image kindly provided by Tulsiram Prathapam, as part of an abreview.Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.
