Anti-Cyclin B1 (phospho S126) antibody (ab55184)
Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S126)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin B1 (phospho S126) antibody
See all Cyclin B1 primary antibodies -
Description
Rabbit polyclonal to Cyclin B1 (phospho S126) -
Host species
Rabbit -
Specificity
ab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Cyclin B1 (phospho S126). Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).
Database link: P14635
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab55184)All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells
treated with EGF (200ng/ml, 15mins).
Lane 2 : extracts from NIH/3T3 cells
treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.
Predicted band size: 48 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S126) antibody (ab55184)Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.
