Anti-Cyclin A2 antibody [E23.1] (ab38)
![Anti-Cyclin A2 antibody [E23.1] (ab38)](https://www.abcam.com/ps/products/0/ab38/Images/ab38-205349-anti-cyclin-a2-antibody-e231-western-blot.jpg "Anti-Cyclin A2 antibody [E23.1] (ab38)")
Key features and details
- Mouse monoclonal [E23.1] to Cyclin A2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Cyclin A2 antibody [E23.1]
See all Cyclin A2 primary antibodies -
Description
Mouse monoclonal [E23.1] to Cyclin A2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Fusion protein corresponding to Cow Cyclin A2.
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Positive control
- In Western Blot, ab38 gavre a positive signal in the following whole cell lysates: HEK293; U20S; PC12; NIH3T3. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
E23.1 -
Myeloma
Sp2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 5 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)](https://www.abcam.com/ps/products/0/ab38/Images/ab38-3248-anti-cyclin-a2-antibody-e231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)IHC image of Cyclin A staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)](https://www.abcam.com/ps/products/0/ab38/Images/ab38-3252-anti-cyclin-a2-antibody-e231-western-blot.jpg)
Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 2 µg/ml
Lane 1 : U2OS (Hu) whole cell lysate
Lane 2 : 293T (Hu) whole cell lysate
Lane 3 : Cos-7 (AGMk) whole cell lysate
Lane 4 : CHO (Hm) whole cell lysate
Lane 5 : PC12 (Rat) whole cell lysate
Lane 6 : 3T3 (Ms) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutesThe membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C.
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![Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)](https://www.abcam.com/ps/products/0/ab38/Images/ab38_1.jpg)
Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38) This image is courtesy of Julian Gannon, CRUK.Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD. Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.
