Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCR2 antibody [EPR22301-103] (ab225732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22301-103] to CXCR2
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CXCR2 antibody [EPR22301-103]
    See all CXCR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22301-103] to CXCR2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human spleen, gastric adenocarcinoma and pancreas adenocarcinoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in neutrophils of human spleen (PMID: 29661142) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)

    Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in stromal cells of human gastric adenocarcinoma (PMID: 28575019) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)

    Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of pancreas tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining inn stromal cells of human adenocarcinoma of pancreas (PMID:27265504) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (ab225732)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control: No staining on human cerebrum (PMID: 28529637) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CXCR2 antibody [EPR22301-103] (ab225732)
    Anti-CXCR2 antibody [EPR22301-103] (ab225732)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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