Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-CXCL9 antibody [EPR23999-5] (ab263442)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23999-5] to CXCL9
Suitable for: Flow Cyt, WB, IP
Reacts with: Human

Product name

Anti-CXCL9 antibody [EPR23999-5]
See all CXCL9 primary antibodies
Description

Rabbit monoclonal [EPR23999-5] to CXCL9
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: THP-1 (treated with 200ng/ml IFN-? and 50 ng/ml LPS(Lipopolysaccharide) for 24 hours) whole cell lysate; His-tagged human purified recombinant full-length protein CXCL9. Flow cyt: THP-1 (treated with 100ng/ml IFN-gamma and 1ug/ml LPS(Lipopolysaccharide) for 48 hours) cells. IP: THP-1 (treated with 100ng/ml IFN-gamma and 1ug/ml LPS(Lipopolysaccharide) for 48 hours) whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23999-5
Isotype

IgG
Research areas

Western blot - Anti-CXCL9 antibody [EPR23999-5] (ab263442)

All lanes : Anti-CXCL9 antibody [EPR23999-5] (ab263442) at 1/1000 dilution

Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 2 : THP-1 (treated with 200 ng /ml IFN-gamma and 50 ng/ml LPS (Lipopolysaccharide) for 24 hours), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 14 kDa
Observed band size: 13,14 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile is consistent with what has been described in the literature (PMID: 15154616, 22973274).

Exposure time: 3 minutes.
Flow Cytometry - Anti-CXCL9 antibody [EPR23999-5] (ab263442)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia monocyte) treated with 100ng/ml IFN-γand 1ug/ml LPS (Lipopolysaccharide) for 48 hours (Red)/ Untreated control (Green) cells labelling CXCL9 with ab263442 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-CXCL9 antibody [EPR23999-5] (ab263442)

CXCL9 was immunoprecipitated from 0.35 mg THP-1(human monocytic leukemia monocyte) (treated with 100ng/ml IFN-γ and 1ug/ml LPS (Lipopolysaccharide) for 48 hours) whole cell lysate with ab263442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263442 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: THP-1 treated with 100ng/ml IFN-γ and 1ug/ml LPS (Lipopolysaccharide) for 48 hours, whole cell lysate 10 ug

Lane 2: ab263442 IP in THP-1 treated with 100ng/ml IFN-γ and 1ug/ml LPS(Lipopolysaccharide) for 48 hours whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263442 in THP-1 treated with 100ng/ml IFN-γ and 1ug/ml LPS(Lipopolysaccharide) for 48 hours whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-CXCL9 antibody [EPR23999-5] (ab263442)

Anti-CXCL9 antibody [EPR23999-5] (ab263442) at 1/1000 dilution + His-tagged human purified recombinant full-length protein CXCL9, 10 ng

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 14 kDa
Observed band size: 13,14 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile is consistent with what has been described in the literature (PMID: 15154616, 22973274).

Exposure time: 5.5 seconds.
Anti-CXCL9 antibody [EPR23999-5] (ab263442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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