Anti-CTRP5 antibody (ab36893)
Key features and details
- Rabbit polyclonal to CTRP5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CTRP5 antibody
See all CTRP5 primary antibodies -
Description
Rabbit polyclonal to CTRP5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 15 amino acid peptide from near the amino terminus of human CTRP5.
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Positive control
- Human brain cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (ab36893) This image is courtesy of an Abreview submitted by Steffen Rickelt
ab36893 staining CTRP5 in human pancreas carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/750 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Western blot - Anti-CTRP5 antibody (ab36893)Lane 1 : Anti-CTRP5 antibody (ab36893) at 1 µg/ml
Lane 2 : Anti-CTRP5 antibody (ab36893) at 2 µg/ml
Lane 3 : Anti-CTRP5 antibody (ab36893) at 4 µg/ml
All lanes : Human brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Lanes read from left to right, 1-3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (ab36893)ab36993 at 10µg/ml staining CTRP5 in human brain tissue by IHC
